His 96-minute speech, which broke the alloted 15-minute protocol, marked the first time he addressed the international body since leading a coup in September 1969 which ended rule by monarchy in Libya and brought him to power.
In an interview with Al Jazeera's Ghida Fakhry on Thursday, Gaddafi repeated his belief that a two-state solution is not a feasible approach to resolving the Israeli-Palestinian conflict and bringing peace in the Middle East.
"Everyone is calling for a two-state solution [but] they don't understand the demographics of the region. Israel would be reduced to 14km and Israel will not accept this," Gaddafi said.
"There is no way to have these two states so close, because they are already integrated. Two million Palestinians live in Israel, Gaza is isolated … torn apart and isolated. Israel should get rid of their nuclear weapons - the Muslim countries will not recognise Israel as long as they have nuclear weapons - and these problems would be done," he added.
"I don't believe that Barack Obama [the US president] really understands the problem," he said.
"He was a senator; he used to worry about the state that he represents and the American people. So he doesn't understand it deeply enough. Maybe in the future he might."
The Libyan leader also said the current Arab approach to resolving the 60-year Arab-Israeli conflict and creating a Palestinian state would not yield positive results.
"There is no way to have two states in that piece of territory."
Source: Al Jazeera (English)